Apo Plasmid EndoFree Midi

Fun iwẹnumọ iyara ti iye alabọde ti pilasima DNA ti ko ni iyasọtọ ti endotoxin.

Apo EndoFree Midi Plasmid gba ilana isọdi awo siliki alailẹgbẹ kan lati ṣe daradara ati pataki dipọ plasmid DNA. EBT Buffer pataki ati Filiration CS1 le yọ awọn idoti ni imunadoko bii endotoxin ati amuaradagba. Gbogbo ilana isediwon gba wakati 1 nikan, eyiti o rọrun ati iyara.

Ologbo. Rara Iṣakojọpọ Iwon
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Apejuwe Ọja

Ṣiṣẹ iṣẹ

Apeere Idanwo

Awọn afi ọja

Awọn ẹya ara ẹrọ

■ Dara fun yiyọ awọn plasmids lati alabọde aṣa kokoro arun 50-100 milimita.
Plas Plasmid ti a ti sọ di mimọ ni endotoxin kekere pupọ ati pe o le pade awọn ibeere ti gbigbe didara ga.
■ Rọrun lati ṣiṣẹ pẹlu ọna alailẹgbẹ ati iyara yiyọ endotoxin.

Awọn ohun elo

DNA plasmid ti a ti sọ di mimọ le ṣee lo ninu awọn adanwo isedale molikula ati awọn adanwo isedale sẹẹli gẹgẹbi ihamọ endonuclease tito nkan lẹsẹsẹ, tito lẹsẹsẹ, iyipada, gbigbe, microinjection, abbl.

Ti nso Plasmid

Yield

Gbogbo awọn ọja le ṣe adani fun ODM/OEM. Fun awọn alaye,jọwọ tẹ Iṣẹ adani (ODM/OEM)


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    Workflow

    Workflow PEGFP-C1 Plasmid jẹ mimọ nipasẹ Apo EndoFree Midi Plasmid lati 50 milimita alabọde kokoro alabọde. Ifojusi pilasima ni a ṣe iwọn nipasẹ Nanodrop ati pe a ṣe iṣiro ikore. Abajade fihan pe ikore plasmid nipasẹ EndoFree Mini Plasmid Kit jẹ ga ju iyẹn nipasẹ ọja ti o yẹ lati Olupese A.
    Workflow PEGFP-C1 plasmid ti a fa jade nipasẹ EndoFree Midi Plasmid Kit ti wa ni gbigbe sinu 80% confluence 293T awọn sẹẹli nipasẹ TIANGEN TIANfect. Ifihan ti GFP ni a rii ni 24-36 h lẹhin gbigbe. Abajade fihan pe ṣiṣe gbigbe jẹ 80%-90%.
    Kọ ifiranṣẹ rẹ nibi ki o firanṣẹ si wa