EndoFree Mini Plasmid Kit II

Fun iwẹnumọ ti DNA pilasima ti ko ni iyasọtọ ti transotoxin.

Apoti Plasmid Mini EndoFree II gba imọ -ẹrọ isọdi awo siliki alailẹgbẹ kan lati mu daradara ati ni pataki dipọ plasmid DNA. Eto yiyọ endotoxin pataki le yọ awọn idoti ni imunadoko bii endotoxin ati awọn ọlọjẹ. Gbogbo ilana isediwon gba wakati 1 nikan, eyiti o rọrun ati iyara. Iyọkuro pilasimidi jẹ ibatan si nọmba ẹda plasmid, iru kokoro arun ti gbalejo ati awọn ipo aṣa. Titi di 70 μg ti DNA plasmid ni a le fa jade lati 5-15 milimita ti alabọde asa awọn kokoro arun ti alẹ.

Ologbo. Rara Iṣakojọpọ Iwon
4992422 50 awọn igbaradi

Apejuwe Ọja

Apeere Idanwo

Awọn afi ọja

Awọn ẹya ara ẹrọ

Dekun ati ikore giga: 5-70 μg DNA plasmid ni a le fa jade ni ayika wakati 1.
Urity Iwa-mimọ: Eto yiyọ endotoxin pataki ni idapo pẹlu Spin Column CP4 le ṣe ifọsi ni pataki nucleic acid ati yọ endotoxin si ipele ti o kere ju.
Range Iwọn awọn ohun elo lọpọlọpọ: Dara fun awọn adanwo igbagbogbo bii ihamọ endonuclease tito nkan lẹsẹsẹ, PCR, tito nkan lẹsẹsẹ, ligation, iyipada, bakanna pẹlu awọn adanwo giga-giga gẹgẹbi itọju jiini, microinjection sẹẹli, ipalọlọ jiini, transcription, abbl.

Ti nso Plasmid

Plasmid Yield

Gbogbo awọn ọja le ṣe adani fun ODM/OEM. Fun awọn alaye,jọwọ tẹ Iṣẹ adani (ODM/OEM)


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    Experimental Example Dara fun isediwon ti awọn plasmids ẹda giga/kekere
    Nọmba 1. Isediwon ti DNA plasmid lati oriṣiriṣi iwọn didun ti alabọde asa kokoro arun nipa lilo EndoFree Mini Plasmid Kit II.
    Iwọn gbigbọn: 200 μl; Iwọn didun ikojọpọ: 3 μl plasmid pbm3 kekere pBR322, 2 μl pBS plasmid giga-daakọ.
    Ti ṣe adaṣe electrophoresis ni 6 V/cm fun iṣẹju 30 lori 1% agarose.
    Gbigbe sẹẹli nipasẹ plasmid ti a ti sọ di mimọ
    Experimental Example Nọmba 2 PlasFP plasmid ti a sọ di mimọ nipasẹ EndoFree Mini Plasmid Kit II ni a ti gbe lọ si awọn sẹẹli 293T. Ifihan ti GFP ni a rii ni 48 h post-transfection
    Kọ ifiranṣẹ rẹ nibi ki o firanṣẹ si wa